Due to the unique reception of the fifth training course on molecular genetics laboratory techniques with the title from gene to protein, for those interested who did not succeed in participating in this course, the sixth training course on molecular genetics laboratory techniques with similar topics and without increasing the price It will be held from 5th to 8th of Bahman this year at the short-term specialized training center of the National Institute of Genetic Engineering and Biotechnology.
In this short-term training course, which is planned for students interested in doing molecular genetics and biotechnology projects and with lower costs than similar courses, common laboratory techniques such as PCR, DNA extraction, enzyme digestion, gene transfer, Electrophoresis, protein extraction, principles of primer design, etc. are taught practically and theoretically, and at the end of the training course, the participants who successfully complete the course will be awarded a valid certificate of the National Institute of Genetic Engineering and Biotechnology.
Workshop program
February 5: PCR training
– Principles of work in the molecular laboratory
– Getting to know the principles of PCR, reaction and PCR program
– Perform PCR
– Different types and applications of PCR
– Basics of electrophoresis and separation of DNA fragments
– Observing PCR products on agarose gel and analyzing the results
– Principles of primer design
6 and 7 Bahman: genetic engineering, DNA cloning
– Principles of genetic engineering and familiarization with the stages of a DNA cloning project
– Applications of genetic engineering
– Cutting enzymes and enzymatic digestion
Ligation reaction and target DNA cloning
– Recombinant DNA transfer to E. Coli recipient bacteria
– Different methods of molecular analysis of cloning accuracy
– Confirmation of cloning with Colony PCR technique
– Electrophoresis and observing the results of recombinant colonies
– DNA extraction using the kit
8 Bahman: protein expression and extraction
– Theoretical principles of working with protein
– Protein extraction
– SDS-PAGE
More about this source textSource text required for additional translation information
Send feedback
Side panels
- mayanshimi
- 124 بازدید